051 The development and functional activities of skin resident memory T cells depends more on fatty-acid binding protein 5 than on fatty-acid binding protein 4

Skin resident memory T cells (TRM) are a subset of which reside in skin for long term. They require exogenous free fatty acid their survival, and fatty-acid binding protein 4 (FABP4) 5 (FABP5) considered to take part lipid uptake. However, the detailed relation FABP4 with TRM function is still unknown. Here, we analyzed role development functional activities murine contact hypersensitivity model. Wild type (WT), knockout (FABP4KO), FABP5 (FABP5KO) mice were sensitized on abdomen 0.5% DNFB at day -7 challenged right ear 0.15% 0. By 28, CD69+CD103+CD8 develop ear. When both ears re-challenged swelling 29 compared left was weakest FABP5KO, followed by FABP4KO WT (0.146 ± 0.007 mm WT, 0.094 0.009 FABP4KO, 0.052 vs FABP4KO: p < 0.001, FABP5KO: 0.05). The number 28 tended be lower FABP5KO than however, difference did not reach significance (484 65 cells/ear 364 41 340 52 = 0.3304, 0.1240, 0.9980). IFNγ production from this fraction comparable while that decreased (104 18 147 19 58 11 =0.2107, 0.2067, Our results imply FABP5, rather FABP4, contributes TRM.